A centrifuge is a vital part of a lab as it is used to separate components of a mixture based on density. Generally, this is essential in terms of research, diagnostics or production. For example, in a clinical setting, a centrifuge could be utilized to separate blood from clotted blood or plasma as part of the testing or analysis process. Pharmaceutically, it can be used to isolate or purify proteins, for nucleic acid extraction, for cell and subcellular component separation and more.
Centrifuges are also key in biotech and work to separate liquid mixtures based on differences in shape, size and density. A centrifuge is a key component in many types of labs and, therefore, must be serviced properly. At Technical Safety Services (TSS), we are fully equipped to partner with you to provide expert calibration and certification of lab equipment. Following outlines more about this unique tool, including calibration, why it matters and step-by-step instructions for calibration.
Why Calibration Matters
In a lab setting for research and more, accuracy is of the utmost importance. Proper calibration of a centrifuge in the calibration process is vital when it comes to accuracy and safe centrifuge operations. When a centrifuge isn’t properly calibrated, it can throw off an entire chain of analysis and can lead to sample damage, irreproducible results or even noncompliance. Speed, time and temperature should also all be on point, as an improperly calibrated machine simply won’t be accurate.
In addition, there are various regulation and accreditation implications to consider, such as ISO, GLP or GMP labs being required to show traceable proof of calibration records. Many industries, such as pharmaceuticals and healthcare, require regular calibration that meets specific standards and regulations like the Current Good Manufacturing Practices (cGMP). When partnering with us at TSS, you can be assured that your centrifuge is ready and is providing accurate and reproducible results as part of your research process. We offer on-site calibration for your centrifuge, complete with documentation that will have you ready for any audit that could come your way. Here’s a quick look at why centrifuge calibration matters so much in virtually any lab setting:
- Accuracy and Reliability: Calibration will ensure that a centrifuge's time, speed and temperature are accurate and trustworthy. Inaccuracy here can skew results.
- Reproducibility and Consistency: Being able to reproduce results over and over is another reason that calibration is so important. When a centrifuge isn’t calibrated properly, it can alter results and lead to unreliable research and data discovery with unreproducible results.
- Regulation Compliance: Many labs are required to show proof of regular calibration in order to meet regulations.
- Safety: A centrifuge that isn’t performing right can even cause some centrifuge safety risks, like imbalance, vibration, sample spillage or rotor failure, which can lead to injury of staff or equipment damage.
- Longevity: Regular calibration is a part of the responsible maintenance for any centrifuge model. It also reduces the overall wear and tear on the centrifuge and helps extend its lifespan and can even reduce repair costs.
How Often to Calibrate
How often you should calibrate your centrifuge will be dependent on usage. High-performance labs should consider twice yearly calibration, while lower-use units can opt for just once a year. At TSS, we recommend a yearly baseline but do encourage more frequent checks for either high-use centrifuges or those that are used in critical processes.
For example, for high-volume clinical or research labs, calibration might instead be needed every three to six months. Of course, you should also consider the regulations overseeing the lab’s practice as well as manufacturer recommendations for that particular machine. Scheduling a calibration should be part of the preventative maintenance plan and is key to a centrifuge’s performance over time.
Step-by-Step Calibration Procedure
When determining how to calibrate a centrifuge, you need to know the following steps. Adhering to these will ensure your machine is running smoothly. At TSS, we are equipped to perform this checklist for you and give you proof of calibration. Following is a look at the step-by-step process involved in properly calibration.
- Preparation & Safety Check
Before doing anything, it’s important to read and familiarize yourself with the manufacturer’s manual. You should also check the rotor condition and the balance. It’s important to understand each machine’s limitations or unique requirements, which are outlined in the manufacturer’s manual as is their preferred calibration procedure. - Leveling & Physical Inspection
Look for evidence of corrosion, cracks or signs of wear on the centrifuge and its components. These defects can negatively impact the balance and RPMs. In some cases, replacing parts should be done before moving to the next step. If the physical inspection is good, then move on to the leveling process. Use a spirit level, also called an inclinometer or bubble level, to perform accurate leveling. - Speed (RPM) Calibration
Speed or RPM is calibrated using either an optical sensor or tachometer. Compare the RPM in terms to what it is set on and the actual RPM. Adjust if outside the tolerance range. - Timer/Duration Check
Next up is timing or duration. Set this by using a stopwatch to verify control panel timing matches the actual run. - Temperature Calibration
Use a calibrated thermometer to check temperature control inside the chamber. - Finalize & Document
Record results, any adjustments made, the technician’s name and the date the service is performed. Of course, at TSS, we can help with each step of this process, certifying equipment and promoting traceability and digital data capture.
Best Practices & Common Pitfalls
Best practices for proper calibration include using certified tools in the hands of trained staff, completing digital logs and creating a consistent schedule for these centrifuge services. Some common pitfalls include skipping rotor inspection, which is key to proper operation. Other common mistakes include calibrating the machine on an uneven surface, which will not allow true and proper balancing. Using an uncertified reference standard and ignoring temperature and timer checks are some others that commonly occur. TSS is here to help you avoid these common pitfalls and ensure your equipment is in optimal working order.
Contact us at TSS
Contact us at TSS to schedule your centrifuge services, including our on-site service, audit-ready results, and full documentation for regulations and accuracy. Regular calibration is a must, not only for consistent results, but also for continued centrifuge safety during operation. Reach out today to learn more about our services and how we can come alongside your lab and help you achieve verifiable and consistent results that can change lives.
