Thermal Cycler, Autoclave

Some of the most powerful tools in the lab today are the devices for genomics and analytical optics. TSS offers the range of services needed for your thermal cycler, spectrophotometer, autoclave and plate reader.

From single-cuvette and single tube cavity to full 96 and 384-well calibrations, we have a plan and service to meet your specific needs.

Scientist is putting ELISA plate to measure OD with micro plate reader, Selective Focus

Thermal cycler calibrations offer the required data for block uniformity to compare to manufacturer’s specifications but also critical overshoot and undershoot information for denaturation and annealing temperatures for the added data to support your PCR processes. The make/model of the device is not a factor in our thermal cycler service, giving the flexibility to compare yields across all brands used in your lab. 

TSS’ ASHI-compliant calibration offers this same information across all 96 wells simultaneously for histocompatibility customers and others with more stringent requirements.

Spectrophotometer calibrations check linearity of absorbance across visible and UV spectra and confirm wavelength accuracy and baseline noise. If your lamp is nearing its end-of-life, we can replace it at a fraction of the cost of the manufacturer, providing the “post-repair” calibration results as a benchmark for optimal performance.

Autoclave calibration ensures the temperature produced by this equipment meets your specifications for sterilization. This typically involves the use of an NIST-traceable standard such as an oil bath or dry block. Our expertise means we can perform this service for optimal accuracy. 

Plate Reader calibration protocols cover fluorescence and luminescence in addition to absorbance, providing complete performance data on multi-mode readers. Whether your reader is a standalone or part of a high-throughput automated liquid handling platform, we provide the peace of mind for this crucial optics platform.

Many of our 400+ nationwide network of technicians have factory training from major OEM’s in the optics and thermal cycler field.

Levels of Support

For additional information about TSS' Thermal Cycler, Plate Reader, Autoclave, and Spectrophotometer Services— and our full suite of services — please call 800-877-7742 or CONTACT the TSS regional office nearest you.

Frequently Asked Questions 

What types of calibration are important for thermal cyclers?

Thermal cycler calibrations include: 

  • Background calibration to ensure the equipment distinguishes fluorescent samples from ambient fluorescence
  • Pure dye spectra calibration that helps the instrument identify the dyes being used 
  • ROI calibration to ensure the software successfully maps positions of the wells
  • Instrument verification to make sure the instrument successfully generates a standard curve

How often should autoclaves be calibrated? 

It is recommended that autoclaves undergo calibration every six to 12 months. This is to verify that the equipment maintains its ability to generate the appropriate temperatures needed for laboratory work. Although these machines are calibrated at the factory, over time they can lose their accuracy through repeated use as well as external factors. 

How often should a spectrophotometer be calibrated?

Due to the highly sensitive nature of this equipment, it is good practice to calibrate this instrument frequently for the best results. Typically, it is recommended that calibration be performed at the start of every project or once every two hours while in use. 

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